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验证数据展示

  • WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)

    WB analysis of Jurkat using 66093-1-Ig (same clone as 66093-1-PBS)

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)

    WB analysis using 66093-1-Ig (same clone as 66093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8/P43/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)

    WB analysis of HeLa cells using 66093-1-Ig (same clone as 66093-1-PBS)

    WB result of Caspase 8/P43/P18 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

    IP experiment of HepG2 using 66093-1-Ig (same clone as 66093-1-PBS)

    IP result of anti-Caspase 8/P43/P18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP8 antibody included
  • IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    IHC staining of human liver cancer using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP8 antibody included
  • IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

    IF Staining of HeLa using 66093-1-Ig (same clone as 66093-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 8/P43/P18 antibody (66093-1-Ig, Clone: 2B9H8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66093-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Caspase 8/P43/P18 Monoclonal antibody, PBS Only

Caspase 8/P43/P18 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66093-1-PBS

产品说明书

CloneNo. 2B9H8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.

产品信息

66093-1-PBS targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Caspase 8/P43/P18 fusion protein Ag20524 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 8, apoptosis-related cysteine peptidase
别名 CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5
计算分子量 538 aa, 62 kDa
观测分子量 53-57 kDa, 32-45 kDa, 18 kDa
GenBank蛋白编号BC028223
基因名称 Caspase 8
Gene ID (NCBI) 841
RRIDAB_11232214
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14790
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.