验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66093-1-Ig targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, monkey, chicken, sheep |
| 免疫原 |
CatNo: Ag20524 Product name: Recombinant human Caspase 8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-270 aa of BC028223 Sequence: MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGTV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | caspase 8, apoptosis-related cysteine peptidase |
| 别名 | CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5 |
| 计算分子量 | 538 aa, 62 kDa |
| 观测分子量 | 53-57 kDa, 32-45 kDa, 18 kDa |
| GenBank蛋白编号 | BC028223 |
| 基因名称 | Caspase 8 |
| Gene ID (NCBI) | 841 |
| RRID | AB_11232214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| IHC protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| IP protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| WB protocol for Caspase 8/P43/P18 antibody 66093-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Targeting PTPN13 with 11-amino-acid peptides of C-terminal APC prevents immune evasion of colorectal cancer. | ||
Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies | ||
Cell Stem Cell Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening | ||
Nat Commun E4F1 coordinates pyruvate metabolism and the activity of the elongator complex to ensure translation fidelity during brain development | ||
J Adv Res Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer | ||
