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Caspase 8/p43/p18 Monoclonal antibody
Caspase 8/p43/p18 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (6)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2B9H8
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 66093-1-Ig is Caspase 8/p43/p18 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP, ELISA |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, chicken, sheep, monkey, pig |
Immunogen | Caspase 8/p43/p18 fusion protein Ag20524 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | caspase 8, apoptosis-related cysteine peptidase |
Synonyms | Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5 |
Calculated Molecular Weight | 538 aa, 62 kDa |
Observed Molecular Weight | 53-57 kDa, 32-45 kDa, 18 kDa |
GenBank Accession Number | BC028223 |
Gene Symbol | CASP8 |
Gene ID (NCBI) | 841 |
RRID | AB_11232214 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14790 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
IHC protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
IF protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
IP protocol for Caspase 8/p43/p18 antibody 66093-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.
| ||
Acta Biomater TRAIL-modified, doxorubicin-embedded periodic mesoporous organosilica nanoparticles for targeted drug delivery and efficient antitumor immunotherapy. | ||
J Pineal Res Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota. | ||
Chem Sci Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay. | ||