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验证数据展示

  • IF Staining of HeLa using CL594-66093

    IF Staining of HeLa using CL594-66093

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66093 (Caspase 8/P43/P18 antibody) at dilution of 1:100.

  • IF Staining of HeLa using CL594-66093

    IF Staining of HeLa using CL594-66093

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Caspase 8/P43/P18 antibody (CL594-66093, Clone: 2B9H8 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Caspase 8/P43/P18 Monoclonal antibody

Caspase 8/P43/P18 Monoclonal Antibody for IF/ICC
Cat No. CL594-66093

产品说明书

CloneNo. 2B9H8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC

别名

CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66093-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66093 targets Caspase 8/P43/P18 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Caspase 8/P43/P18 fusion protein Ag20524 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase 8, apoptosis-related cysteine peptidase
别名 CASP8, Caspase 8, 2B9H8, Apoptotic cysteine protease, Apoptotic protease Mch-5
计算分子量 538 aa, 62 kDa
观测分子量 53-57 kDa, 32-45 kDa, 18 kDa
GenBank蛋白编号BC028223
基因名称 Caspase 8
Gene ID (NCBI) 841
RRIDAB_2883508
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14790
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

实验方案

Product Specific Protocols
IF protocol for CL594 Caspase 8/P43/P18 antibody CL594-66093Download protocol
Standard Protocols
Click here to view our Standard Protocols