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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Caspase 8/p43/p18 Monoclonal antibody

Caspase 8/p43/p18 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B9H8

Cat no : CL488-66093

Print datasheet

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

验证数据展示

  • IF Staining of HeLa using CL488-66093

    IF Staining of HeLa using CL488-66093

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66093 (Caspase 8/p43/p18 antibody) at dilution of 1:100.

  • IF Staining of HeLa using CL488-66093

    IF Staining of HeLa using CL488-66093

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 8/p43/p18 antibody (CL488-66093, Clone: 2B9H8 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66093-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66093 targets Caspase 8/p43/p18 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Caspase 8/p43/p18 fusion protein Ag20524 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caspase 8, apoptosis-related cysteine peptidase
Synonyms CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5
Calculated Molecular Weight 538 aa, 62 kDa
Observed Molecular Weight 53-57 kDa, 32-45 kDa, 18 kDa
GenBank Accession NumberBC028223
Gene Symbol Caspase 8
Gene ID (NCBI) 841
RRIDAB_2883255
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14790
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 8/p43/p18 antibody CL488-66093Download protocol
Standard Protocols
Click here to view our Standard Protocols