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ChromoTek CoraLite® Plus 647-conjugated LAG3 VHH

CL647-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-lt

Synonyms

LAG3-Nanobody, LAG3-single domain antibody (sdAb), CD223, FDC, LAG 3, LAG3, LAG-3, lymphocyte activation gene 3, Protein FDC

Validation Data Gallery

  • 1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt). Cells were not fixed.

    1X10^6 HEK LAG3 transient transfected cells (red) and HEK parental cells were surface stained with 0.25 µg CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt). Cells were not fixed.

  • Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt, 1:500, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL647-lt can also be performed after formaldehyde fixation of the cells.

    Live HeLa cells transfected with human LAG3 were immunostained with CoraLite® Plus 647 conjugated-LAG3 VHH (CL647-lt, 1:500, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.  Note: Immunostaining with CL647-lt can also be performed after formaldehyde fixation of the cells.

  • Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

    Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated LAG-3 VHH (CL647-lt) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

Product Information

CL647-lt targets LAG3 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 647 Fluorescent Dye
Type Nanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.141 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
RRIDAB_3101918
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C; Shipped at ambient temperature.
BackgroundLAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)