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DACH1 Monoclonal antibody
DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
3B6D2
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, C6 cells, L02 cells, ROS1728 cells, HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human endometrial cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue, mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy DACH1 Ready-To-Use IHC Kit. DACH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60082-1-Ig targets DACH1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DACH1 fusion protein Ag4474 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dachshund homolog 1 (Drosophila) |
| Synonyms | Dachshund-1, DACH, 3B6D2 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 97-110 kDa |
| GenBank Accession Number | BC021219 |
| Gene Symbol | DACH1 |
| Gene ID (NCBI) | 1602 |
| RRID | AB_2089711 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UI36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DACH1 antibody 60082-1-Ig | Download protocol |
| IHC protocol for DACH1 antibody 60082-1-Ig | Download protocol |
| IF protocol for DACH1 antibody 60082-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling | ||
Cell Biol Int DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma | ||
Front Oncol Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas
| ||
Clin Exp Metastasis Lung-derived soluble factors support stemness/plasticity and metastatic behaviour of breast cancer cells via the FGF2-DACH1 axis |
