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  • Featured Product
  • KD/KO Validated

DACH1 Monoclonal antibody, PBS Only

DACH1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3B6D2

Cat No : 60082-1-PBS

Print datasheet

Synonyms

DACH, DACH1, Dachshund homolog 1

验证数据展示

  • WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

产品信息

60082-1-PBS targets DACH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity human, rat, mouse
Immunogen DACH1 fusion protein Ag4474 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dachshund homolog 1 (Drosophila)
Synonyms DACH, DACH1, Dachshund homolog 1
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UI36
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.