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  • Featured Product
  • KD/KO Validated

DACH1 Monoclonal antibody, PBS Only

DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B6D2

Cat no : 60082-1-PBS

Print datasheet

Synonyms

Dachshund-1, DACH, 3B6D2

验证数据展示

  • WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DACH1 antibody included
  • IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DACH1 Ready-To-Use IHC Kit. DACH1 primary antibody included.

产品信息

60082-1-PBS targets DACH1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DACH1 fusion protein Ag4474 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dachshund homolog 1 (Drosophila)
Synonyms Dachshund-1, DACH, 3B6D2
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2089711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UI36
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.