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验证数据展示

  • WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DACH1 antibody included
  • IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DACH1 Monoclonal antibody, PBS Only

DACH1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60082-1-PBS

产品说明书

CloneNo. 3B6D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Dachshund-1, DACH, 3B6D2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DACH1 Ready-To-Use IHC Kit. DACH1 primary antibody included.

产品信息

60082-1-PBS targets DACH1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4474

Product name: Recombinant human DACH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 176-521 aa of BC021219

Sequence: QHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRVETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPKEGDLAGHDMGHESKRMHIEKDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSIETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAKRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dachshund homolog 1 (Drosophila)
别名 Dachshund-1, DACH, 3B6D2
计算分子量 79 kDa
观测分子量 97-110 kDa
GenBank蛋白编号BC021219
基因名称 DACH1
Gene ID (NCBI) 1602
RRIDAB_2089711
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UI36
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

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