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ChromoTek DNMT1-Trap Agarose

The ChromoTek DNMT1-Trap Agarose consists of an anti-DNMT1 Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of DNMT1 proteins and their interacting factors.

Specificity

DNMT1

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Cat No : dta

Synonyms

DNMT1, DNA methyltransferase, AIM, CXXC9, HSN1E, DNMT, DNMT1 Trap

Validation Data Gallery

  • The DNMT1-Trap Agarose was used to immunoprecipiate endogenous DNMT1 protein from HEK293T cells. Input (I), Flow-Through (FT), Bound B).

    The DNMT1-Trap Agarose was used to immunoprecipiate endogenous DNMT1 protein from HEK293T cells. Input (I), Flow-Through (FT), Bound B).

  • IP of endogenous DNMT1 protein from HEK293T cells using DNMT-1 Trap Agarose followed by western blot using DNMT1 Polyclonal Antibody (24206-1-AP) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (RGAR001). Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the western blot analysis.

    IP of endogenous DNMT1 protein from HEK293T cells using DNMT-1 Trap Agarose followed by western blot using DNMT1 Polyclonal Antibody (24206-1-AP) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (RGAR001). Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the western blot analysis.

Product Information

The ChromoTek DNMT1-Trap Agarose consists of an anti-DNMT1 Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of DNMT1 proteins and their interacting factors.

DescriptionImmunoprecipitation of DNMT1 proteins with anti-DNMT1 Nanobody conjugated to agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, Co-IP
Specificity/TargetDNMT1 protein
Binding Capacity2-3 ug of DNMT1 protein per 10 uL bead slurry
ConjugateAgarose beads; ~90 um (cross-linked 4% agarose beads)
Elution buffer2x SDS-sample buffer (Lammli)
TypeNanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe DNMT1-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_2631372
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Nat Struct Mol Biol

Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

Authors - Julio C Y Liu
View Article
IP

Nat Commun

Inhibition of TOPORS ubiquitin ligase augments the efficacy of DNA hypomethylating agents through DNMT1 stabilization

Authors - Satoshi Kaito
View Article