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DOK4 Polyclonal antibody

DOK4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10481-2-AP

Print datasheet

Synonyms

docking protein 4, DOK4, IRS 5, IRS5

验证数据展示

  • WB analysis using 10481-2-AP

    WB analysis using 10481-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10481-2-AP (DOK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10481-2-AP targets DOK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DOK4 fusion protein Ag0734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name docking protein 4
Synonyms docking protein 4, DOK4, IRS 5, IRS5
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC003541
Gene Symbol DOK4
Gene ID (NCBI) 55715
RRIDAB_2246205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The INS-receptor substrate family plays important roles in cellular growth, signaling and survival. IRS5/DOK4 is a new member of this family identified, which is known as docking protein 4, or downstream of tyrosine kinase-4. DOK4 might have roles in INS and IGF-1 signaling as well. Expression of IRS5/DOK4 is regulated epigenetically via histone acetylating at its promoter. DOK4 is required at early stages in the myelination process, including the initial interaction with the axon, and is also involved in Schwann cell migration and proliferation, it also regulates GDNF-mediated neurite outgrowth during neuronal development through activation of the Rap1-ERK1/2 pathway. DOK4 may represent a novel negative regulator of T cells as other DOK family members like DOK1 and DOK2.

实验方案

Product Specific Protocols
WB protocol for DOK4 antibody 10481-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols