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验证数据展示

  • WB analysis using 82805-1-RR

    WB analysis using 82805-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82805-1-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82805-1-RR

    Affinity and Kinetic Characterization of 82805-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82805-1-RR against Human GRP94 were performed. The affinity constant is 1.30 nM.

GRP94 Recombinant antibody

GRP94 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82805-1-RR

产品说明书

CloneNo. 6K5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82805-1-RR targets GRP94 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 GRP94 fusion protein Ag6344 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock protein 90kDa beta (Grp94), member 1
别名 HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
计算分子量 92 kDa
观测分子量 94-100 kDa
GenBank蛋白编号BC066656
基因名称 GRP94
Gene ID (NCBI) 7184
RRIDAB_3086543
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14625
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRP94 antibody 82805-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols