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ChromoTek GST-Trap Agarose, kit

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Specificity

GST

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat No : stak

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase

Validation Data Gallery

  • Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

    Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

DescriptionThe GST-Trap Agarose Kit contains GST-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GST-fusion proteins and their interacting factors.

• Effectively immunoprecipitates low expressed GST-fusion proteins

• Considerably higher affinity than glutathione cellulose and other affinity resins

• Strong binding even under harsh washing conditions

• No heavy & light chains in your downstream application

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetGlutathione S-Transferase (GST) from Schistosoma japonicum
Binding capacity7.5-10 μg of recombinant GST per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 nM
Compatibility with mass spectrometryThe GST-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631414
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
GST-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Other Formats

GST-Trap Agarose