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ChromoTek HA-Trap Magnetic Particles M-270 Kit

The HA-Trap Magnetic Particles M-270 Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to magnetic particles M-270, along with lysis, wash, and elution buffers to use in the IP process. It is highly recommended when very large proteins/complexes are being investigated.

Specificity

HA-Tag (sequence YPYDVPDYA)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat No : atdk

Synonyms

Hemagglutinin tag, HA, HA-tag, HA-tag Trap Magnetic Particles

Validation Data Gallery

  • The HA-Trap Magnetic Particles M-270 Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

    The HA-Trap Magnetic Particles M-270 Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

  • WB detection of TOM70-HA fusion protein following immunoprecipitation with HA-Trap Magnetic Particles M-270 Kit from either untransfected (mock) HEK293T cells or HEK293T cells transfected with full-length TOM70-HA construct. Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the WB analysis. Detection was completed using TOM70 Monoclonal Antibody (66593-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001).

    WB detection of TOM70-HA fusion protein following immunoprecipitation with HA-Trap Magnetic Particles M-270 Kit from either untransfected (mock) HEK293T cells or HEK293T cells transfected with full-length TOM70-HA construct. Samples from the Input (I), Flow-Through (F), and Bound (B) fractions were used in the WB analysis. Detection was completed using TOM70 Monoclonal Antibody (66593-1-Ig) and Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM001).

Product Information

The HA-Trap Magnetic Particles M-270 Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to magnetic particles M-270, along with lysis, wash, and elution buffers to use in the IP process. It is highly recommended when very large proteins/complexes are being investigated.

DescriptionThe HA-Trap Magnetic Particles M-270 Kit contains HA-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of HA-tagged proteins.

• Fast, reliable & efficient one-step immunoprecipitation without size limitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for automated and high throughput applications

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
Binding Capacity5 ug of recombinant HA-tagged protein (~30 kDA) per 25 uL bead slurry
ConjugateMagnetic Particles, M-270, size: 2.8 μm
Elution buffer2x SDS-sample buffer (Lammli)
Wash Buffer Compatibility<1 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0 % SDS, 1 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions.
Compatibility with mass spectrometryThe HA-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3094562
Storage BufferPBS with 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component Description
HA-Trap Magnetic Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution