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HDAC3 Recombinant antibody, PBS Only

HDAC3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2N1

Cat no : 81211-1-PBS

Print datasheet

Synonyms

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

验证数据展示

  • WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

    WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • Affinity and Kinetic Characterization of 81211-1-PBS

    Affinity and Kinetic Characterization of 81211-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81211-1-RR against Human HDAC3 were performed. The affinity constant is 0.213 nM.

Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

产品信息

81211-1-PBS targets HDAC3 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen HDAC3 fusion protein Ag0396 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone deacetylase 3
Synonyms HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC000614
Gene Symbol HDAC3
Gene ID (NCBI) 8841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15379
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.