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ChromoTek His Fab-Trap® Magnetic Particles M-270

The ChromoTek His Fab-Trap® Magnetic Particles M-270 consists of an anti-His-tag Fab fragment, which is coupled to magnetic particles. It can be used for the immunoprecipitation of proteins fused to the His-tag from cell extracts of various organisms. It is highly recommended when very large proteins/complexes are being investigated.

Specificity

6*His, His-Tag (sequence: HHHHHH)

Applications

IP, Co-IP

Type

Fab-fragment

Conjugate

Magnetic Particles M-270

Cat No : hfd

Synonyms

His-trap, 6*His Fab-Trap, His-tag Fab-Trap

Validation Data Gallery

  • IP using His Fab-Trap® Magnetic Particles M-270 (hfd) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

    IP using His Fab-Trap® Magnetic Particles M-270 (hfd) from untransfected HEK293T (mock) and HEK293T cells transfected with either His-TOM70 or TOM70-His fusion proteins. WB detection of TOM70 protein was also done on samples from the Input (I), Flow-Through (F), and Bound (B) fractions using Anti-TOM70 Polyclonal Antibody (14528-1-AP) and ChromoTek Nano-Secondary alpaca anti-rabbit IgG, CoraLite Plus 488 (srb2GCL488-1).

Product Information

The ChromoTek His Fab-Trap® Magnetic Particles M-270 consists of an anti-His-tag Fab fragment, which is coupled to magnetic particles. It can be used for the immunoprecipitation of proteins fused to the His-tag from cell extracts of various organisms. It is highly recommended when very large proteins/complexes are being investigated.

DescriptionImmunoprecipitation of His-tagged proteins with anti-His-tag Fab-fragment conjugated to magnetic particles.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• Low background

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the His-tag (also known as hexahistidine or His6-tag) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion (dissociation constant KD of 10 nM for a C-terminal His-tag and 220 nM for an N-terminal His-tag).
Binding Capacity1 μg of recombinant His-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270; size: 2.8 µM
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 or 100 µM His-Peptide
Wash Buffer Compatibility2 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, <0.1% SDS, 3-4 M Urea
TypeFab-fragment
ClassRecombinant
HostMouse
Affinity (KD)10 nM for a C-terminal His-tag and 220 nM for a N-terminal His-tag
Compatibility with mass spectrometryThe His Fab-Trap® is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3665061
Storage BufferPBS pH 7.4. Preservative: 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!