Human/Rat BDNF ELISA Kit
Cat no : KE00096
Synonyms
BDNF precursor form, brain derived neurotrophic factor, Brain-derived neurotrophic factor
Validation Data Gallery
Product Information
KE00096 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BDNF ELISA kit is to be used to detect and quantify protein levels of endogenous BDNF. The assay recognizes human BDNF. An antibody specific for BDNF has been pre-coated onto the microwells. The BDNF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BDNF is added to detect the captured human BDNF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Rat BDNF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Rat serum, Rat plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 12.5 - 800 pg/mL |
| Reactivity | Human/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 627 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 92% | 74%-117% |
| Cell culture supernatants | 90% | 73%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 35.8 | 2.3 | 6.3 |
| 2 | 20 | 116.5 | 9.2 | 7.9 |
| 3 | 20 | 513.9 | 36.9 | 7.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 32.2 | 2.6 | 8.1 |
| 2 | 24 | 108.6 | 8.2 | 7.6 |
| 3 | 24 | 517.3 | 40.8 | 7.9 |
Background Information
The brain-derived neurotrophic factor (BDNF), as a member of the neurotrophin family of growth factors, is broadly expressed in the central nervous system. BDNF is involved in cerebral morphogenesis, synaptic plasticity and learning, mental health, degenerative central nervous system (CNS) diseases, and energy metabolism. BDNF has recently been identified as one of the four major pharmaceutical targets in neuropsychiatric diseases, including depression and bipolar disorder. In addition, BDNF is increasingly considered as a potential therapeutic agent for neurodevelopmental disorders such as Rett syndrome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BDNF precursor form, brain derived neurotrophic factor, Brain-derived neurotrophic factor |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Geroscience A novel synthetic 3,4,5-tri-feruloylquinic acid enhances learning and memory via neurotrophin signaling in an aging model senescence-accelerated prone 8 mice | ||
Front Cell Dev Biol Luteolin Modulates Neural Stem Cells Fate Determination: In vitro Study on Human Neural Stem Cells, and in vivo Study on LPS-Induced Depression Mice Model. | ||
Korean J Anesthesiol Intrathecal dexmedetomidine attenuates mechanical allodynia through the downregulation of brain-derived neurotrophic factor in a mild traumatic brain injury rat. | ||
Front Mol Neurosci Kif21B mediates the effect of estradiol on the morphological plasticity of mouse hippocampal neurons | ||
Int J Mol Sci Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression. | ||
Dose Response Intraoperative Dexmedetomidine in Peripheral or Emergency Neurologic Surgeries of Patients With Mild-to-Moderate Traumatic Brain Injuries: A Retrospective Cohort Study. |
