AuthentiKine™ Human IL-10 ELISA Kit
Validation Data Gallery
Product Information
KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-10 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3586 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human plasma | 92% | 79%-100% |
| Cell culture supernatants | 102% | 88%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 67.8 | 4.5 | 6.7 |
| 2 | 20 | 175.0 | 10.0 | 5.7 |
| 3 | 20 | 926.2 | 42.2 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 193.9 | 17.6 | 9.1 |
| 2 | 24 | 432.4 | 38.9 | 9.0 |
| 3 | 24 | 1,108.4 | 111.3 | 10.0 |
Background Information
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF |
Publications
| Species | Sample Type | Title |
|---|---|---|
Mol Ther Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells. | ||
Front Cell Infect Microbiol The effect of gut microbiota dysbiosis on patients with preeclampsia | ||
Front Immunol Regulatory B Cells Dysregulated T Cell Function in an IL-35-Dependent Way in Patients With Chronic Hepatitis B. | ||
Front Immunol Circulating Type I Interferon Levels in the Early Phase of COVID-19 Are Associated With the Development of Respiratory Failure. | ||
Biochem Biophys Res Commun Hesperidin inhibits methylation and autophagy in LPS and high glucose-induced human villous trophoblasts | ||
J Biomed Mater Res B Appl Biomater Macrophage activation in response to shape memory polymer foam-coated aneurysm occlusion devices. |