Human/Mouse SMN ELISA Kit
Sensitivity
6.0 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human/Mouse
Cat no : KE00027
Synonyms
Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMNT, Survival motor neuron protein
Validation Data Gallery
Product Information
KE00027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SMN ELISA kit is designed for the quantitative determination of SMN in samples of human and mouse origin. An antibody specific for SMN has been pre-coated onto the microwells. The SMN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SMN is added to detect the captured SMN protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse SMN ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 6.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6607 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 101% | 83%-127% |
| Tissue lysate | 93% | 71%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,037.6 | 63.2 | 6.1 |
| 2 | 20 | 1,678.6 | 71.1 | 4.2 |
| 3 | 20 | 2,465.5 | 98.0 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,219.4 | 93.9 | 7.7 |
| 2 | 24 | 2,594.4 | 152.6 | 5.9 |
| 3 | 24 | 4,296 | 252.7 | 5.9 |
Background Information
Survival Motor Neuron (SMN) protein required for efficient assembly of small nuclear ribonucleoprotein (snRNP) complexes is encoded by nearly identical telomeric and centromeric forms of SMN gene respectively. Both the SMN1 and SMN2 genes express SMN protein, however, the amount of functional full-length protein produced by SMN2 is much less than that produced by SMN1 due to the alternative splicing.The SMN gene is constitutively expressed in wide variety of tissues including brain, kidney, liver and spinal cord, while motor neurons are particularly vulnerable to reduced SMN protein levels. Deletion or mutational inactivation of the SMN1 gene causes spinal muscular atrophy (SMA), a lethal genetic disease characterized by loss of motor neurons in the spinal cord. The absence of SMN1 can be partially compensated for by SMN2 and the SMN2 expression level is associated with SMA severity. This kit is for the quantitative determination of SMN protein level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMNT, Survival motor neuron protein |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
