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IFT88 Recombinant antibody

IFT88 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230074E12

Cat no : 82886-2-RR

Print datasheet

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

验证数据展示

  • WB analysis using 82886-2-RR

    WB analysis using 82886-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 82886-2-RR

    IF Staining of hTERT-RPE1 using 82886-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • FC experiment of HEK-293 using 82886-2-RR

    FC experiment of HEK-293 using 82886-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82886-2-RR

    Affinity and Kinetic Characterization of 82886-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, PC-3 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82886-2-RR targets IFT88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen IFT88 fusion protein Ag4980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Synonyms TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 88-95 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).

实验方案

Product Specific Protocols
WB protocol for IFT88 antibody 82886-2-RRDownload protocol
IF protocol for IFT88 antibody 82886-2-RRDownload protocol
FC protocol for IFT88 antibody 82886-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols