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  • Featured Product
  • KD/KO Validated

LONP1 Polyclonal antibody

LONP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15440-1-AP

Print datasheet

Synonyms

PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON

验证数据展示

  • WB analysis of Jurkat cells using 15440-1-AP

    WB analysis of Jurkat cells using 15440-1-AP

    WB result of LONP1 antibody (15440-1-AP, 1:6000) with si-Control and si-LONP1 transfected Jurkat cells.

  • WB analysis using 15440-1-AP

    WB analysis using 15440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 15440-1-AP

    IP experiment of mouse heart using 15440-1-AP

    IP result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.

  • IHC staining of human lung cancer using 15440-1-AP

    IHC staining of human lung cancer using 15440-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15440-1-AP

    IHC staining of human lung cancer using 15440-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15440-1-AP

    WB analysis using 15440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15440-1-AP

    IF Staining of HepG2 using 15440-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 15440-1-AP

    IF Staining of HepG2 using 15440-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 15440-1-AP

    IF Staining of HepG2 using 15440-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15440-1-AP targets LONP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, chicken
Immunogen LONP1 fusion protein Ag7196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2137152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

实验方案

Product Specific Protocols
WB protocol for LONP1 antibody 15440-1-APDownload protocol
IHC protocol for LONP1 antibody 15440-1-APDownload protocol
IF protocol for LONP1 antibody 15440-1-APDownload protocol
IP protocol for LONP1 antibody 15440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
  • KD Validated
View Article
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
View Article
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
View Article
humanWB

Mol Cell

Circadian Oscillation of Sulfiredoxin in the Mitochondria.

Authors - In Sup Kil
  • KD Validated
View Article
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
View Article
mouseWB,IHC

Sci Adv

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Authors - Qiqi Guo
View Article