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验证数据展示

  • IHC staining of human skin using 13662-1-AP

    IHC staining of human skin using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 13662-1-AP

    IHC staining of human skin using 13662-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of HEK-293 using 13662-1-AP

    WB analysis of HEK-293 using 13662-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13662-1-AP

    IP experiment of HEK-293 using 13662-1-AP

    IP result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.

LTA4H Polyclonal antibody

LTA4H Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13662-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

Leukotriene A 4 hydrolase, Leukotriene A(4) hydrolase, leukotriene A4 hydrolase, LTA 4 hydrolase, LTA4, LTA4H

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13662-1-AP targets LTA4H in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 LTA4H fusion protein Ag4568 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leukotriene A4 hydrolase
别名 Leukotriene A 4 hydrolase, Leukotriene A(4) hydrolase, leukotriene A4 hydrolase, LTA 4 hydrolase, LTA4, LTA4H
计算分子量 611 aa, 69 kDa
观测分子量 57-70 kDa
GenBank蛋白编号BC032528
基因名称 LTA4H
Gene ID (NCBI) 4048
RRIDAB_2138747
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09960
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

实验方案

Product Specific Protocols
WB protocol for LTA4H antibody 13662-1-APDownload protocol
IHC protocol for LTA4H antibody 13662-1-APDownload protocol
IP protocol for LTA4H antibody 13662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Signal

ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis

Authors - Hao Cai
View Article
humanWB

Proteomics

Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.

Authors - Wei Gao
View Article
mouseIF

JHEP Rep

Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction

Authors - Gan Chen
View Article