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MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12508-1-AP

Print datasheet

Synonyms

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

验证数据展示

  • WB analysis using 12508-1-AP

    WB analysis using 12508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 12508-1-AP

    FC experiment of A431 using 12508-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug MAGEA4 Polyclonal antibody (12508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human breast cancer using 12508-1-AP

    IHC staining of human breast cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inA431 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MAGEA4 fusion protein Ag3193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family A, 4
Synonyms MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_2877863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 12508-1-APDownload protocol
IHC protocol for MAGEA4 antibody 12508-1-APDownload protocol
FC protocol for MAGEA4 antibody 12508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment

Authors - Yunpeng Wang
View Article