MAGEA4 Polyclonal antibody
MAGEA4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, U2OS cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
12508-1-AP targets MAGEA4 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | MAGEA4 fusion protein Ag3193 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | melanoma antigen family A, 4 |
Synonyms | Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4 |
Calculated Molecular Weight | 317 aa, 35 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC017723 |
Gene Symbol | MAGEA4 |
Gene ID (NCBI) | 4103 |
RRID | AB_2877863 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P43358 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
IHC protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Endocrinol (Lausanne) Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment |