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验证数据展示

  • WB analysis using 12508-1-AP

    WB analysis using 12508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12508-1-AP

    IHC staining of human lung cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 12508-1-AP

    FC experiment of A431 using 12508-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug MAGEA4 Polyclonal antibody (12508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human breast cancer using 12508-1-AP

    IHC staining of human breast cancer using 12508-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 12508-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 MAGEA4 fusion protein Ag3193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family A, 4
别名 MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4
计算分子量 317 aa, 35 kDa
观测分子量 45 kDa
GenBank蛋白编号BC017723
基因名称 MAGEA4
Gene ID (NCBI) 4103
RRIDAB_2877863
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43358
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 12508-1-APDownload protocol
IHC protocol for MAGEA4 antibody 12508-1-APDownload protocol
FC protocol for MAGEA4 antibody 12508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Endocrinol (Lausanne)

Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment

Authors - Yunpeng Wang
View Article