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CoraLite® Plus 488-conjugated MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12508

Print datasheet

Synonyms

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

验证数据展示

  • FC experiment of A431 using CL488-12508

    FC experiment of A431 using CL488-12508

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAGEA4 Polyclonal antibody (CL488-12508)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 12508-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12508 targets MAGEA4 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen MAGEA4 fusion protein Ag3193 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family A, 4
Synonyms MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_2919055
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43358
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MAGEA4 antibody CL488-12508Download protocol
Standard Protocols
Click here to view our Standard Protocols