ChromoTek MK2-Trap Agarose, kit

ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

Specificity

MK2

Applications

IP, CoIP

Conjugate

Agarose

Cat No : mtak

Synonyms

MK2-Trap, MK2, MAPKAPK2, MAPKAP kinase 2, MAPKAPK 2



Product Information

ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

ApplicationsIP, CoIP
ClonalityMonoclonal
HostAlpaca
Specificity/TargetMK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2)
Species-Reactivity: Tested on human, mouse, hamster
Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
Coupled VHHRecombinant, monoclonal anti-MK2 single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
MK2-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution