ChromoTek MK2-Trap Agarose, kit
ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.
Specificity
MK2
Applications
IP, CoIP
Conjugate
Agarose
Cat No : mtak
Synonyms
Validation Data Gallery
Product Information
ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.
| Applications | IP, CoIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Specificity/Target | MK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2) Species-Reactivity: Tested on human, mouse, hamster Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested. |
| Coupled VHH | Recombinant, monoclonal anti-MK2 single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| MK2-Trap Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |