Validation Data Gallery
Product Information
ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.
| Description | The MK2-Trap Agarose Kit contains MK2-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of MK2.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Short incubation time (1 h at 4°C) |
| Applications | IP, CoIP |
| Specificity/Target | MK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2) |
| Tested Reactivity | Human, Mouse, Hamster Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested. |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The MK2-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631376 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| MK2-Trap Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |