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验证数据展示

  • IF Staining of HepG2 using 81515-2-RR

    IF Staining of HepG2 using 81515-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (81515-2-RR, Clone: 240131F5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 81515-2-RR

    FC experiment of HeLa using 81515-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP2 Recombinant antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HT-29 using 81515-2-RR

    FC experiment of HT-29 using 81515-2-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug MMP2 Recombinant Antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81515-2-RR

    Affinity and Kinetic Characterization of 81515-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81515-2-RR against Human MMP2 were performed. The affinity constant is below 1 pM.

MMP2 Recombinant antibody

MMP2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 81515-2-RR

产品说明书

CloneNo. 240131F5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHT-29 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81515-2-RR targets MMP2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MMP2 fusion protein Ag0549 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
别名 Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase
计算分子量 72 kDa
观测分子量 62-72 kDa
GenBank蛋白编号BC002576
基因名称 MMP2
Gene ID (NCBI) 4313
RRIDAB_3670498
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP08253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

实验方案

Product Specific Protocols
IF protocol for MMP2 antibody 81515-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols