MMP2 Recombinant antibody
MMP2 Recombinant Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240131F5
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 81515-2-RR is MMP2 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | MMP2 fusion protein Ag0549 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
Synonyms | 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1 |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 62-72 kDa |
GenBank Accession Number | BC002576 |
Gene Symbol | MMP2 |
Gene ID (NCBI) | 4313 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P08253 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).
实验方案
Product Specific Protocols | |
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IF protocol for MMP2 antibody 81515-2-RR | Download protocol |
FC protocol for MMP2 antibody 81515-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |