MYL12B Polyclonal antibody
MYL12B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissues |
Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IF | See 1 publications below |
产品信息
10324-1-AP targets MYL12B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | MYL12B fusion protein Ag0398 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | myosin, light chain 12B, regulatory |
Synonyms | SHUJUN-1, SHUJUN 1, Myosin regulatory light chain 2-B, smooth muscle isoform, MYLC2B, MRLC2 |
Calculated Molecular Weight | 20 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC004994 |
Gene Symbol | MYL12B |
Gene ID (NCBI) | 103910 |
RRID | AB_2166830 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14950 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MYL12B antibody 10324-1-AP | Download protocol |
IHC protocol for MYL12B antibody 10324-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Biol Cell Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. | ||
Biochemistry (Mosc) Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells. | ||
BMC Cell Biol Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. | ||
J Pept Sci Design of peptidase-resistant peptide inhibitors of myosin light chain kinase. | ||
Biochemistry (Mosc) Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro |