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MultiPro® 5CFLX Anti-Human Lamin B1 (3C10G12)

Lamin B1 Monoclonal Antibody for Single Cell (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

TCGTACTATACTAAC

CloneNo.

3C10G12

Cat no : G66095-1-5C

Print datasheet

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

验证数据展示

  • Single Cell Sequencing experiment G66095-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66095-1-5C on Resting PBMC

    G66095-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66095-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66095-1-5C on PBMC treated with 4hr LPS + TI

    G66095-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66095-1-5C targets Lamin B1 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Lamin B1 fusion protein Ag20522 种属同源性预测
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human Lamin B1 (3C10G12)
Synonyms ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
Calculated Molecular Weight 66 kDa
GenBank Accession NumberBC012295
Gene Symbol Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGTCGTACTATACTAACCCCATATAAGAAA
Barcode SequenceTCGTACTATACTAAC
Form Liquid
UNIPROT IDP20700
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol