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NCAM1/CD56 Monoclonal antibody, PBS Only

NCAM1/CD56 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E8C9

Cat no : 60238-1-PBS

Print datasheet

Synonyms

CD56, NCAM1, 1E8C9, NCAM 1, NCAM

验证数据展示

  • WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD56/NCAM1 Ready-To-Use IHC Kit. CD56/NCAM1 primary antibody included.

产品信息

60238-1-PBS targets NCAM1/CD56 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen NCAM1/CD56 fusion protein Ag5732 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, NCAM1, 1E8C9, NCAM 1, NCAM
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).