验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20018-1-AP targets NCOR1 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear receptor co-repressor 1 |
| 别名 | hCIT529I10, hN CoR, KIAA1047, N CoR, N CoR1, NCOR1, Nuclear receptor corepressor 1, TRAC1 |
| 计算分子量 | 270 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_006311 |
| 基因名称 | NCOR1 |
| Gene ID (NCBI) | 9611 |
| RRID | AB_10665360 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75376 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| IHC protocol for NCOR1 antibody 20018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease | ||
PLoS Biol Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock. | ||
iScience WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation | ||
Theranostics Palmitoylation of PKCδ by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease | ||
Cell Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor | ||
Cell Mol Life Sci Novel role of NCoR1 in impairing spatial memory through the mediation of a novel interacting protein DEC2
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