ChromoTek Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, for 2x Cys conjugation [CTK0114]
Monovalent, recombinant secondary single domain antibody to mouse IgG2a: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
Applications
IF, WB, FC
Reactivity
mouse IgG2a
Host
Alpaca
Conjugate
Unconjugated
Cat no : smsG2aCys2-1
Synonyms
Validation Data Gallery
![The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114]
has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.](/products/pictures/Model-smsG2aCys2-1.jpg "The alpaca anti-mouse IgG2a Fc-specific recombinant VHH [CTK0114]
has a very high affinity (KD <10 pM) for mouse IgG2a primary antibody.")
Product Information
Monovalent, recombinant secondary single domain antibody to mouse IgG2a: alpaca monoclonal Nanobody, Fc-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides
| Applications | IF, WB, FC |
| Host | Alpaca-derived, recombinantly produced in bacteria |
| Species Reactivity | Mouse IgG2a Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2b, IgG2c, IgG3 |
| Target/Specificity | Fc-fragment of mouse IgG2a |
| Physical State | Liquid |
| Conjugate | Unconjugated |
| Molecular weight | 14.4 kDa |
| Extinction coefficient (280 nm) | 24,200 M-1*cm-1 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Secondary Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG1, IgG2b, IgG2c, and IgG3 |
| Immunogen | Purified mouse IgG2a |
| Clonality | Monoclonal Nanobody |
| Clones | CTK0114 |
| Affinity (KD) | KD <10 pM |
| RRID | AB_2935700 |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C. |
| Size | 500 µg |
| Note | This product is for research use only, not for diagnostic or therapeutic use |