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验证数据展示

  • WB analysis using 83600-2-RR (same clone as 83600-2-PBS)

    WB analysis using 83600-2-RR (same clone as 83600-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83600-2-RR (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83600-2-PBS in a different storage buffer formulation.

  • IF Staining of MDA-MB-231 using 83600-2-RR (same clone as 83600-2-PBS)

    IF Staining of MDA-MB-231 using 83600-2-RR (same clone as 83600-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PD-L1/CD274 antibody (83600-2-RR, Clone: 240721E12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83600-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00601-1

    Cytometric bead array standard curve of MP00601-1

    Cytometric bead array standard curve of MP00601-1, PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83600-2-PBS. Detection antibody: 83600-5-PBS. Standard: Eg1114. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83600-2-RR

    Affinity and Kinetic Characterization of 83600-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83600-2-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.275 nM.

PD-L1/CD274 Recombinant antibody, PBS Only (Capture)

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83600-2-PBS

产品说明书

CloneNo. 240721E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD274, PD-L1, PD L1, hPD-L1, B7-H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83600-2-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00601-1: 83600-2-PBS capture and 83600-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD274 molecule
别名 CD274, PD-L1, PD L1, hPD-L1, B7-H1
计算分子量 290 aa, 33 kDa
GenBank蛋白编号BC074984
基因名称 PD-L1
Gene ID (NCBI) 29126
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZQ7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C