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PD-L1/CD274 Recombinant antibody, PBS Only (Capture)

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240721E12

Cat no : 83600-2-PBS

Print datasheet

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

验证数据展示

  • WB analysis using 83600-2-RR (same clone as 83600-2-PBS)

    WB analysis using 83600-2-RR (same clone as 83600-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83600-2-RR (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83600-2-PBS in a different storage buffer formulation.

  • IF Staining of MDA-MB-231 using 83600-2-RR (same clone as 83600-2-PBS)

    IF Staining of MDA-MB-231 using 83600-2-RR (same clone as 83600-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PD-L1/CD274 antibody (83600-2-RR, Clone: 240721E12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83600-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00601-1

    Cytometric bead array standard curve of MP00601-1

    Cytometric bead array standard curve of MP00601-1, PD-L1/CD274 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83600-2-PBS. Detection antibody: 83600-5-PBS. Standard: Eg1114. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83600-2-RR

    Affinity and Kinetic Characterization of 83600-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83600-2-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.275 nM.

产品信息

83600-2-PBS targets PD-L1/CD274 as part of a matched antibody pair:

MP00601-1: 83600-2-PBS capture and 83600-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 molecule
Synonyms CD274, PD-L1, PD L1, hPD-L1, B7-H1
Calculated Molecular Weight 290 aa, 33 kDa
GenBank Accession NumberBC074984
Gene Symbol PD-L1
Gene ID (NCBI) 29126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZQ7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C