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验证数据展示

  • WB analysis using 67910-1-Ig

    WB analysis using 67910-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human colon cancer using 67910-1-Ig

    IHC staining of human colon cancer using 67910-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA1 antibody included
  • IHC staining of human colon cancer using 67910-1-Ig

    IHC staining of human colon cancer using 67910-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA1 antibody included

PSMA1 Monoclonal antibody

PSMA1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67910-1-Ig

产品说明书

CloneNo. 1D1D5

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, RAW 264.7 cells, 4T1 cells, Hela cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA1 Ready-To-Use IHC Kit. PSMA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67910-1-Ig targets PSMA1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PSMA1 fusion protein Ag30527 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 1
别名 30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1
计算分子量 30 kDa
观测分子量 29-33 kDa
GenBank蛋白编号BC015105
基因名称 PSMA1
Gene ID (NCBI) 5682
RRIDAB_2918665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).

实验方案

Product Specific Protocols
WB protocol for PSMA1 antibody 67910-1-IgDownload protocol
IHC protocol for PSMA1 antibody 67910-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols