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PSMD7 Monoclonal antibody

PSMD7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E10E7

Cat no : 68188-1-Ig

Print datasheet

Synonyms

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

验证数据展示

  • WB analysis using 68188-1-Ig

    WB analysis using 68188-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HeLa using 68188-1-Ig

    WB analysis of HeLa using 68188-1-Ig

    WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD7 Ready-To-Use IHC Kit. PSMD7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68188-1-Ig targets PSMD7 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen PSMD7 fusion protein Ag9305 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Synonyms MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012606
Gene Symbol PSMD7
Gene ID (NCBI) 5713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

实验方案

Product Specific Protocols
WB protocol for PSMD7 antibody 68188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Authors - Di Wang
View Article