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验证数据展示

  • WB analysis using 68188-1-Ig

    WB analysis using 68188-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of HeLa using 68188-1-Ig

    WB analysis of HeLa using 68188-1-Ig

    WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

PSMD7 Monoclonal antibody

PSMD7 Monoclonal Antibody for WB, ELISA
Cat No. 68188-1-Ig

产品说明书

CloneNo. 3E10E7
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, ELISA

别名

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD7 Ready-To-Use IHC Kit. PSMD7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68188-1-Ig targets PSMD7 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 PSMD7 fusion protein Ag9305 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
别名 MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12
计算分子量 324 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC012606
基因名称 PSMD7
Gene ID (NCBI) 5713
RRIDAB_2935278
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51665
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

实验方案

Product Specific Protocols
WB protocol for PSMD7 antibody 68188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Authors - Di Wang
View Article