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验证数据展示

  • WB analysis using 29110-1-AP

    WB analysis using 29110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29110-1-AP (RAB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 29110-1-AP

    IHC staining of human liver cancer using 29110-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 29110-1-AP

    IHC staining of human liver cancer using 29110-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAB20 Polyclonal antibody

RAB20 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29110-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

FLJ20429, RAB20, Ras related protein Rab 20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29110-1-AP targets RAB20 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen RAB20 fusion protein Ag30459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB20, member RAS oncogene family
Synonyms FLJ20429, RAB20, Ras related protein Rab 20
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC026025
Gene Symbol RAB20
Gene ID (NCBI) 55647
RRIDAB_3086099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB20 is a member of RAS oncogene family, it has no close relationship to any other members of RAB subfamily which consists of small GTP-binding proteins involved in the regulation of intracellullar vesicular transport. Firstly found highly expressed on the apical side of kidney tubuel epithelial cells, RAB20 was speculated to be involved in apical endocytosi and recylcing processes. So far, its function is not well known, however, RAB20 is recently found higher expressed in pancreatic adenocarcinomas than normal pancreas, which is proved by WB and IHC analyses, suggesting its promising role in diagnosis of pancreatic carcinogenesis. Catalog#11616-1-AP is a rabbit polyclonal antibody raised against full-length of human RAB20.

实验方案

Product Specific Protocols
WB protocol for RAB20 antibody 29110-1-APDownload protocol
IHC protocol for RAB20 antibody 29110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols