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验证数据展示

  • WB analysis using 29110-1-AP

    WB analysis using 29110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29110-1-AP (RAB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 29110-1-AP

    IHC staining of human liver cancer using 29110-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 29110-1-AP

    IHC staining of human liver cancer using 29110-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29110-1-AP (RAB20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAB20 Polyclonal antibody

RAB20 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29110-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

FLJ20429, RAB20, Ras related protein Rab 20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29110-1-AP targets RAB20 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 RAB20 fusion protein Ag30459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB20, member RAS oncogene family
别名 FLJ20429, RAB20, Ras related protein Rab 20
计算分子量 26 kDa
观测分子量 29 kDa
GenBank蛋白编号BC026025
基因名称 RAB20
Gene ID (NCBI) 55647
RRIDAB_3086099
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX57
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB20 is a member of RAS oncogene family, it has no close relationship to any other members of RAB subfamily which consists of small GTP-binding proteins involved in the regulation of intracellullar vesicular transport. Firstly found highly expressed on the apical side of kidney tubuel epithelial cells, RAB20 was speculated to be involved in apical endocytosi and recylcing processes. So far, its function is not well known, however, RAB20 is recently found higher expressed in pancreatic adenocarcinomas than normal pancreas, which is proved by WB and IHC analyses, suggesting its promising role in diagnosis of pancreatic carcinogenesis. Catalog#11616-1-AP is a rabbit polyclonal antibody raised against full-length of human RAB20.

实验方案

Product Specific Protocols
WB protocol for RAB20 antibody 29110-1-APDownload protocol
IHC protocol for RAB20 antibody 29110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols