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RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20228-1-AP

Print datasheet

Synonyms

RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

验证数据展示

  • WB analysis of human brain using 20228-1-AP

    WB analysis of human brain using 20228-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20228-1-AP

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20228-1-AP

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 20228-1-AP

    IP experiment of mouse brain using 20228-1-AP

    IP result of anti-RAB5A-Specific (IP:20228-1-AP, 4ug; Detection:20228-1-AP 1:600) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue, mouse colon tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB5A, member RAS oncogene family
Synonyms RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberNM_004162
Gene Symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_10694147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20339
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

实验方案

Product Specific Protocols
WB protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IHC protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IP protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Physiol

Overexpression of ameloblastin in secretory ameloblasts results in demarcated, hypomineralized opacities in enamel

Authors - Yong-Hee Patricia Chun
View Article