验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, A375 cells, Raji cells, HepG2 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, human lung tissue, human brain tissue, human kidney tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14363-1-AP targets RBM3 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, squirrel |
| 免疫原 | RBM3 fusion protein Ag5934 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding motif (RNP1, RRM) protein 3 |
| 别名 | RNPL, RNA-binding protein 3, RNA-binding motif protein 3, RNA binding motif protein 3, Putative RNA binding protein 3 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17-20 kDa |
| GenBank蛋白编号 | BC006825 |
| 基因名称 | RBM3 |
| Gene ID (NCBI) | 5935 |
| RRID | AB_2269266 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P98179 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM3 antibody 14363-1-AP | Download protocol |
| IHC protocol for RBM3 antibody 14363-1-AP | Download protocol |
| IF protocol for RBM3 antibody 14363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.
| ||
Cell iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. | ||
EBioMedicine The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production. | ||
Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing | ||
J Cell Biol Cold-induced protein RBM3 orchestrates neurogenesis via modulating Yap mRNA stability in cold stress.
| ||
Front Cell Dev Biol The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia. |
