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ChromoTek RFP-Booster ATTO594

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

ATTO 594

Host

Alpaca

Cat No : rba594

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

Validation Data Gallery

  • Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.

    Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.

Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateATTO 594
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:200
Type Nanobody
ClassRecombinant
RRIDAB_2631390
Affinity (KD) Dissociation constant KD of 5nM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Science

SIGNAL TRANSDUCTION. Membrane potential modulates plasma membrane phospholipid dynamics and K-Ras signaling.

Authors - Yong Zhou
View Article
IF

Cell

Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Authors - Sahana Holla
View Article
IF

Cell

Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body.

Authors - Chenchen Pan
View Article
IF

Nature

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Authors - Marina Vietri
View Article
IF

Nat Neurosci

Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections.

Authors - Ruiyao Cai
View Article
IF

Nat Cell Biol

Drosophila patterning is established by differential association of mRNAs with P bodies.

Authors - Timothy T Weil
View Article