验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55160-1-AP targets RPGRIP1L in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, zebrafish, canine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RPGRIP1-like |
| 别名 | CORS3, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L |
| 计算分子量 | 151 kDa |
| GenBank蛋白编号 | NM_015272 |
| 基因名称 | RPGRIP1L |
| Gene ID (NCBI) | 23322 |
| RRID | AB_10860269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q68CZ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5). This antibody is specific to RPGRIP1L.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| IHC protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| IP protocol for RPGRIP1L antibody 55160-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Am J Hum Genet Mutations in CSPP1, Encoding a Core Centrosomal Protein, Cause a Range of Ciliopathy Phenotypes in Humans. | ||
J Cell Biol TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone. | ||
Dev Cell Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia. |
