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FSP1/S100A4 Recombinant antibody

FSP1/S100A4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241448A12

Cat no : 84221-1-RR

Print datasheet

Synonyms

S100A4, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL

验证数据展示

  • WB analysis using 84221-1-RR

    WB analysis using 84221-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84221-1-RR (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84221-1-RR

    IF Staining of HeLa using 84221-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (84221-1-RR, Clone: 241448A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84221-1-RR

    FC experiment of HeLa using 84221-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Jurkat using 84221-1-RR

    FC experiment of Jurkat using 84221-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84221-1-RR

    Affinity and Kinetic Characterization of 84221-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84221-1-RR against Human S100A4 were performed. The affinity constant is 2.75 nM.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A4 Ready-To-Use IHC Kit. S100A4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84221-1-RR is FSP1/S100A4 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FSP1/S100A4 fusion protein Ag9019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A4
Synonyms S100A4, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL
Calculated Molecular Weight 101 aa, 12 kDa
Observed Molecular Weight 10-12 kDa
GenBank Accession NumberBC016300
Gene Symbol S100A4
Gene ID (NCBI) 6275
ENSEMBL Gene IDENSG00000196154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

实验方案

Product Specific Protocols
WB protocol for FSP1/S100A4 antibody 84221-1-RRDownload protocol
IF protocol for FSP1/S100A4 antibody 84221-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols