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SF3B3 Monoclonal antibody, PBS Only

SF3B3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7F5

Cat no : 67469-1-PBS

Print datasheet

Synonyms

SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017

验证数据展示

  • WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

    WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67469-1-Ig (SF3B3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

    IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

    IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

    IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SF3B3 Ready-To-Use IHC Kit. SF3B3 primary antibody included.

产品信息

67469-1-PBS targets SF3B3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SF3B3 fusion protein Ag6315 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name splicing factor 3b, subunit 3, 130kDa
Synonyms SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017
Calculated Molecular Weight 136 kDa
Observed Molecular Weight 130-135 kDa
GenBank Accession NumberBC003146
Gene Symbol SF3B3
Gene ID (NCBI) 23450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15393
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.