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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

    WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67469-1-Ig (SF3B3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

    IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

    IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

    IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

SF3B3 Monoclonal antibody, PBS Only

SF3B3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67469-1-PBS

产品说明书

CloneNo. 2H7F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SF3B3 Ready-To-Use IHC Kit. SF3B3 primary antibody included.

产品信息

67469-1-PBS targets SF3B3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SF3B3 fusion protein Ag6315 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 splicing factor 3b, subunit 3, 130kDa
别名 SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017
计算分子量 136 kDa
观测分子量 130-135 kDa
GenBank蛋白编号BC003146
基因名称 SF3B3
Gene ID (NCBI) 23450
RRIDAB_2882698
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15393
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.