ChromoTek SNAP/CLIP-tag-Trap Agarose beads, kit

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

Specificity

SNAP/CLIP-tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Cat No : wtak

Synonyms

SNAP/CLIP-tag-Trap, SNAP/CLIP-tag, SNAP/CLIP, SNAP, human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT), SNAP-tag and CLIP-tag, SNAP-tag, CLIP-tag, CLIP



Product Information

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

DescriptionThe SNAP/CLIP-tag-Trap Agarose Kit contains SNAP/CLIP-tag-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SNAP-tag and CLIP-tag fusion proteins and their interacting factors.

• SNAP/CLIP-tag-Trap immunoprecipitates SNAP-tag- and Clip-tag- fusion proteinseven when already covalently bound to ligands such as dyes, biotin etc.

• Bound SNAP- or CLIP-tag fusion proteins can be eluted without protease

• SNAP/CLIP-tag-Trap can be used for affinity purification

• Structure and function are characterized

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSNAP-tag, CLIP-tag
Binding capacity7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 nM
Compatibility with mass spectrometryThe SNAP/CLIP-tag®-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827598
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
SNAP/CLIP-tag-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution