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  • Featured Product
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STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 10253-2-AP

Print datasheet

Synonyms

STAT 3, HIES, APRF, Acute-phase response factor

验证数据展示

  • WB analysis using 10253-2-AP

    WB analysis using 10253-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10253-2-AP

    IF Staining of HeLa using 10253-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

  • IHC staining of human stomach cancer using 10253-2-AP

    IHC staining of human stomach cancer using 10253-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT3 antibody included
  • IP experiment of HeLa using 10253-2-AP

    IP experiment of HeLa using 10253-2-AP

    IP result of anti-STAT3 (IP:10253-2-AP, 3ug; Detection:10253-2-AP 1:2000) with HeLa cells lysate 3000ug.

  • WB analysis of A549 using 10253-2-AP

    WB analysis of A549 using 10253-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10253-2-AP

    WB analysis using 10253-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10253-2-AP

    WB analysis of A549 using 10253-2-AP

    WB result of STAT3 antibody (10253-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected A549 cells.

  • WB analysis using 10253-2-AP

    WB analysis using 10253-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10253-2-AP

    IF Staining of HeLa using 10253-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 10253-2-AP

    IF Staining of HeLa using 10253-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed IFN alpha treated HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • FC experiment of Ramos using 10253-2-AP

    FC experiment of Ramos using 10253-2-AP

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human STAT3 (10253-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, mouse colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, IFN alpha treated HeLa cells
Positive FC (Intra) detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10253-2-AP targets STAT3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, FC (Intra), IP, CoIP, ChIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, bovine, marmoset
Immunogen STAT3 fusion protein Ag0360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms STAT 3, HIES, APRF, Acute-phase response factor
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
RRIDAB_2302876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

实验方案

Product Specific Protocols
WB protocol for STAT3 antibody 10253-2-APDownload protocol
IHC protocol for STAT3 antibody 10253-2-APDownload protocol
IF protocol for STAT3 antibody 10253-2-APDownload protocol
IP protocol for STAT3 antibody 10253-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

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ACS Appl Mater Interfaces

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