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TMPO/LAP2 Polyclonal antibody
TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue, mouse lung tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TMPO Ready-To-Use IHC Kit. TMPO primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14651-1-AP targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Immunogen | TMPO/LAP2 fusion protein Ag6247 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | thymopoietin |
| Synonyms | LAP2, TMPO, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopoietin-related peptide isoforms beta/gamma, Thymopoietin isoform alpha |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 75 kDa, 51 kDa and 39 kDa |
| GenBank Accession Number | BC053675 |
| Gene Symbol | TMPO/LAP2 |
| Gene ID (NCBI) | 7112 |
| RRID | AB_2205440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42167 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| IHC protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| IF protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| IP protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. | ||
Sci Rep MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells. | ||
J Dairy Sci Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.
| ||
World J Surg Oncol Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.
| ||
Theranostics Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane. |
