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TSR2 Polyclonal antibody

TSR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16263-1-AP

Print datasheet

Synonyms

WGG1, Pre-rRNA-processing protein TSR2 homolog, DT1P1A10

验证数据展示

  • WB analysis of HeLa using 16263-1-AP

    WB analysis of HeLa using 16263-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16263-1-AP

    IHC staining of human liver using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16263-1-AP

    IHC staining of human liver using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 16263-1-AP

    IF Staining of MCF-7 using 16263-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TSR2 antibody (16263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman liver tissue, human epididymis tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

16263-1-AP targets TSR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TSR2 fusion protein Ag9328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
Synonyms WGG1, Pre-rRNA-processing protein TSR2 homolog, DT1P1A10
Calculated Molecular Weight 191 aa, 21 kDa
Observed Molecular Weight 24-30 kDa
GenBank Accession NumberBC011825
Gene Symbol TSR2
Gene ID (NCBI) 90121
RRIDAB_2211381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSR2 antibody 16263-1-APDownload protocol
IHC protocol for TSR2 antibody 16263-1-APDownload protocol
IF protocol for TSR2 antibody 16263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Acta Histochem

Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.

Authors - Ekaterina S Snigirevskaya
View Article
humanIHC

Cell Biol Int

Ultrastructural traits of apoptosis

Authors - Ekaterina S Snigirevskaya
View Article