Alpha Tubulin Polyclonal antibody
Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Immunogen | Alpha Tubulin fusion protein Ag6038 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | tubulin, alpha 1a |
| Synonyms | acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC050637 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2212258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q71U36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| IHC protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| IF protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells | ||
mBio Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner | ||
J Neurosci Res Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion. | ||
J Cell Sci ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments. | ||
Biochim Biophys Acta Mol Basis Dis Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E. | ||
Exp Cell Res Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. |
