Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 14555-1-AP

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Synonyms

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain

验证数据展示

WB analysis of mouse brain using 14555-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 14555-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14555-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 14555-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 14555-1-AP

Size

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经过测试的应用

Positive WB detected in	mouse brain tissue, mouse cerebellum tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 11 publications below
IHC	See 1 publications below
IF	See 1 publications below

产品信息

14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, ELISA Application Description
Cited Applications	WB, IHC, IF
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	Alpha Tubulin fusion protein Ag6038 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	tubulin, alpha 1a
Synonyms	acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, EC:3.6.5.-, Detyrosinated tubulin alpha-1A chain
Calculated Molecular Weight	50 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC050637
Gene Symbol	Alpha Tubulin
Gene ID (NCBI)	7846
RRID	AB_2212258
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q71U36
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol
IHC protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol
IF protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	J Neurosci Res Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion. Authors - Shaqayeq Roqanian View Article
human	WB	J Cell Sci ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments. Authors - Mohamed Bourmoum View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E. Authors - Yan Wang View Article
human	WB,IF	Exp Cell Res Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. Authors - Shen Enzhi E View Article
human	WB	Cell Rep SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation. Authors - Shufen Li View Article
human	WB	Nanomedicine Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer. Authors - Adel Al Fatease View Article

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Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

验证数据展示

WB analysis of mouse brain using 14555-1-AP

IHC staining of mouse testis using 14555-1-AP

IF Staining of HeLa using 14555-1-AP

IF Staining of HeLa using 14555-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.

ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.

Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.