Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14555-1-AP

Print datasheet

Synonyms

alpha tubulin, TUBA1A, TUBA3



经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

14555-1-AP targets Alpha Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag6038
Full Name tubulin, alpha 1a
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC050637
Gene symbol TUBA1A
UniprotQ71U36
Gene ID (NCBI) 7846
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 14555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nanomedicine

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.

Authors - Adel Al Fatease
humanWB

Cell Rep

SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.

Authors - Shufen Li
humanWB,IF

Exp Cell Res

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.

Authors - Shen Enzhi E
mouseWB

Biochim Biophys Acta Mol Basis Dis

Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.

Authors - Yan Wang
humanWB

J Cell Sci

ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.

Authors - Mohamed Bourmoum
mouseWB

J Neurosci Res

Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.

Authors - Shaqayeq Roqanian