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YAP1 Monoclonal antibody
YAP1 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IF/ICC, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
3A7A9
验证数据展示
经过测试的应用
Positive WB detected in | NCI-H1299 cells, HUVEC cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, MCF-7 cells, LNCaP cells, HEK-293 cells, HepG2 cells, NIH-3T3 cells, 4T1 cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66900-1-Ig targets YAP1 in WB, IP, IHC, CoIP, ELISA, IF/ICC applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | YAP1 fusion protein Ag28194 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | Yes-associated protein 1, 65kDa |
Synonyms | 65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |
Calculated Molecular Weight | 504 aa, 54 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC038235 |
Gene Symbol | YAP1 |
Gene ID (NCBI) | 10413 |
RRID | AB_2882229 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P46937 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for YAP1 antibody 66900-1-Ig | Download protocol |
IHC protocol for YAP1 antibody 66900-1-Ig | Download protocol |
IF protocol for YAP1 antibody 66900-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Commun Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation | ||
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
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Cancer Lett A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation
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Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Oncogene Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.
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Acta Pharmacol Sin Critical role of PAFR/YAP1 positive feedback loop in cardiac fibrosis.
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