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ChromoTek anti-CD16 recombinant VHH, for 2x Cys conjugation

CD16Cys2 is an unconjugated recombinant anti CD16 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat No : CD16Cys2

Synonyms

CD16 VHH, CD16 single domain antibody (sdAb), FCGR3, IGFR3, Fc-gamma R3, FcRIII

Validation Data Gallery

  • BLI analysis of the interaction between varying concentrations of anti-CD16 VHH and immobilised, biotinylated CD16A. Fit indicates fitting of data to a 1:1 binding model. Binding curves are similar for CD16B.

    BLI analysis of the interaction between varying concentrations of anti-CD16 VHH and immobilised, biotinylated CD16A. Fit indicates fitting of data to a 1:1 binding model. Binding curves are similar for CD16B.

Product Information

CD16Cys2 is an unconjugated recombinant anti CD16 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity2 nM for CD16A, 4 nM for CD16B
Molecular Weight15.6 kDa
FormLiquid
RRIDAB_3665401
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingdry ice
BackgroundCD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.