calreticulin Recombinant antibody
calreticulin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2B17
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human thyroid cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CALR Ready-To-Use IHC Kit. CALR primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80017-1-RR targets calreticulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | calreticulin fusion protein Ag26064 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | calreticulin |
| Synonyms | CALR, Calregulin, 2B17 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002500 |
| Gene Symbol | Calreticulin |
| Gene ID (NCBI) | 811 |
| RRID | AB_2882941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P27797 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for calreticulin antibody 80017-1-RR | Download protocol |
| IHC protocol for calreticulin antibody 80017-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
