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calreticulin Recombinant antibody

calreticulin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat No : 80017-1-RR

Print datasheet

Synonyms

CALR, Calregulin, 2B17

验证数据展示

  • WB analysis using 80017-1-RR

    WB analysis using 80017-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 80017-1-RR

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 80017-1-RR

    IHC staining of human thyroid cancer using 80017-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity experiments of 80017-1-RR

    Affinity experiments of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman thyroid cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80017-1-RR targets calreticulin in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen calreticulin fusion protein Ag26064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calreticulin
Synonyms CALR, Calregulin, 2B17
Calculated Molecular Weight 60 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC002500
Gene Symbol Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for calreticulin antibody 80017-1-RRDownload protocol
IHC protocol for calreticulin antibody 80017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols