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验证数据展示

  • WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • Affinity and Kinetic Characterization of 80017-1-RR

    Affinity and Kinetic Characterization of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

calreticulin Recombinant antibody, PBS Only

calreticulin Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80017-1-PBS

产品说明书

CloneNo. 2B17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CALR, Calregulin, 2B17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CALR Ready-To-Use IHC Kit. CALR primary antibody included.

产品信息

80017-1-PBS targets calreticulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 calreticulin fusion protein Ag26064 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calreticulin
别名 CALR, Calregulin, 2B17
计算分子量 60 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC002500
基因名称 Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27797
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.