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calreticulin Recombinant antibody, PBS Only

calreticulin Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat no : 80017-1-PBS

Print datasheet

Synonyms

CALR, Calregulin, 2B17

验证数据展示

  • WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    WB analysis using 80017-1-RR (same clone as 80017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80017-1-RR (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    IHC staining of human thyroid cancer using 80017-1-RR (same clone as 80017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80017-1-RR (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALR antibody included
  • Affinity and Kinetic Characterization of 80017-1-RR

    Affinity and Kinetic Characterization of 80017-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80017-1-RR against Human calreticulin were performed. The affinity constant is 6.07 nM.

Planning an IHC experiment? We recommend our IHCeasy CALR Ready-To-Use IHC Kit. CALR primary antibody included.

产品信息

80017-1-PBS targets calreticulin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen calreticulin fusion protein Ag26064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calreticulin
Synonyms CALR, Calregulin, 2B17
Calculated Molecular Weight 60 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC002500
Gene Symbol Calreticulin
Gene ID (NCBI) 811
RRIDAB_2882941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27797
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.