• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Human APOL1 ELISA Kit

Recently cited in top scientific publications as a useful tool regarding HIV, CKD, & FTD studies.

View the APOL1 product page
ELISA Technical Tips

APOL1 Background

APOL1 is involved in the formation and efflux of cholesteryl esters, and also lipid transport. Recent studies have shown that alteration in APOL1 function can lead to obesity, chronic kidney disease (CKD), schizophrenia, or cancer (1 and 2).

Proteintech offers a human-specific APOL1 ELISA kit, with high sensitivity (0.07 nm/ml) and broad assay range (0.156–10 ng/ml). The KE00047 kit is a quantitative measurement of human APOL1 protein in serum and plasma. It has recently been cited in top scientific publications as a useful tool regarding HIV, CKD, or frontotemporal dementia (FTD) studies (3-5).

Performing ELISA experiments, Lan and colleagues reported that HIV infection enhanced APOL1 expression in human vascular smooth muscle cells (SMCs) (3).

APOL1 is also involved in the pathology of FTD, which primarily affects the frontal and temporal lobes of the brain. Teunissen et al. evaluated APOL1 levels in the cerebrospinal fluid (CSF) of FTD patients, performing proteomic analysis to screen proteins with different expression levels, which were later validated by ELISA assays. One of their discoveries was that FTD-tau patients had one of the highest APOL1 fold changes when compared to other FTD subtypes (4).

A recent paper from Nature Medicine relates to the pathogenesis of APOL1-associated kidney nephropathy and its soluble urokinase plasminogen activator receptor (suPAR). Higher suPAR levels were linked to a greater decline in kidney function. The in vitro and in vivo experiments describe clinical and molecular interactions between suPAR and APOL1, suggesting both to be novel and strong predictors of kidney disease. The nephropathologic effect of APOL1 based on suPAR-mediated αvβ3-integrin activation could be a safe therapeutic approach to the treatment of CKD. Serum APOL1 obtained from blood samples was measured using a human Proteintech APOL1 ELISA kit (5).

Proteintech’s APOL1 ELISA kit is specific for the detection of human APOL1. No cross-reactivity to APOL1 analogs has been found.

Catalog number Sample Types: Distribution:
KE00047 Serum, plasma Worldwide
Assay Type: Sensitivity: Precision:
Sandwich ELISA 0.07 ng/mL Intra-assay CV - 3.4%
Reactivity: Range: Intra-assay CV - 3.6%
Human 0.156-10 ng/mL Regulatory:
Tests: Assay time: Research use only
1 X 96 well plate 3.5h  

Recovery

Sample Type Average Range
Plasma 94% 87% - 107%

Standard Curve

Human APOL1 ELISA kit four parameter logistic curve fit

Browse the full ELISA kit catalog here


References

  1. APOL1 nephropathy: from gene to mechanisms of kidney injury.
  2. Human Apolipoprotein L1 (ApoL1) in Cancer and Chronic Kidney Disease (Review Paper)
  3. Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu.
  4. Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics.
  5. A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease.