Human APOL1 ELISA Kit
Validation Data Gallery
Product Information
KE00047 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOL1 ELISA kit is to be used to detect and quantify protein levels of endogenous APOL1. The assay recognizes human APOL1. An antibody specific for APOL1 has been pre-coated onto the microwells. The APOL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for APOL1 is added to detect the captured APOL1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APOL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8542 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 105% | 83%-127% |
| Human plasma | 93% | 85%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.31 | 0.312 | 3.8 |
| 2 | 20 | 2.21 | 0.095 | 4.3 |
| 3 | 20 | 0.63 | 0.013 | 2.1 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.12 | 0.387 | 4.2 |
| 2 | 24 | 2.31 | 0.073 | 3.2 |
| 3 | 24 | 0.62 | 0.023 | 3.6 |
Background Information
Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles. The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. This kit is used to quantify APOL1 level.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | APOL, APOL1, Apolipoprotein L, Apolipoprotein L1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Med A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease. | ||
Immunity APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis. | ||
Alzheimers Dement (Amst) Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics. | ||
Sci Rep Circulating Apolipoprotein L1 is associated with insulin resistance-induced abnormal lipid metabolism. | ||
Exp Mol Pathol Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu. | ||
JCI Insight Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice. |
